This rabbit polyclonal antiserum specifically targets the vesicular acetylcholine transporter, serving as a unique immunohistochemical marker for cholinergic nerves. Unlike common markers like acetylcholinesterase (AchE) and choline acetyltransferase (ChAT), it does not react with postsynaptic neurons, making it more specific. It also offers greater sensitivity. Notably, this antiserum recognizes VAChT in both the central nervous system (CNS) and peripheral nervous system (PNS).