Dialyzed Premium Fetal Bovine Serum, US Origin, is employed in applications needing serum devoid of small molecular weight compounds like nucleosides and amino acids, crucial for alternative biochemical survival routes. The dia-filtration process yields consistent results, reducing hypoxanthine and thymidine levels below detectable limits. Throughout, the glucose level, indicative of dialysis extent, is monitored. The process concludes when the glucose level falls below 5mg/dL.