Mimic a variety of in vivo conditions with 2 membrane materials and 4 porosities Inserts center in wells, which allows pipette access to lower chamber, and prevents wicking between insert and side wall Inserts hang above tissue culture-treated wells, allowing co-culture; grow cells on both the insert membrane and the well bottom 0.1μm and 0.4μm PET inserts feature clear membranes for improved cell viewing PC membrane is best for cell attachment; PET is best for imaging and microscopy 0.1μm and 0.4μm pore sizes are ideal for cell-cell interaction and drug transport studies 3.0μm and 8.0μm pore sizes are ideal for angiogenesis, migration, and chemotaxis experiments