Start your newly captured queen off right in this starting nest. The easiest way to start your colony is in a test tube. To make your experience easier and more enjoyable we created these nifty feeding troughs. Just swap them out as you change out the food. Having an undisturbed chamber is also important for your ants to stay calm as your replace food. Our easily removable camber divider works perfectly to separate the feeding area from the founding chamber. Just fill the test tube a quarter the way with water and insert the cotton ball down enough to remove air the water side. Air expands and contracts with heat which could push water past the cotton ball. Dab up any remaining water drops in the new founding chamber. Install chamber divided with pull tab facing outward and used the feeding trough to push into final position. Add your captured queen ant and carefully plug the the end with the venting plug. You can change to placement of the chamber divider as needed. We included an extra feeding trough and plug to switch out when changing food. Included: 1X Test Tube Holder 1X 20mm OD Glass Test tube 1X Chamber Divider 2X Feeding Troughs 2X Venting Plugs 1X Sliding Cover