Dissolve bFGF and EGF in 0.1% BSA solution (KnockOut D-MEM/F-12) at 100 μg/mL
Combine components in a sterile environment
Preserve unused portions in aliquots
Prepare coating matrix for culture vessels
Cultivate and passage human NSCs
Cultivate and passage rat NSCs
Description
Neural stem cells (NSCs), prized for their potential to transform into neurons and glial cells, are crucial in neuroscience and clinical treatment of neurodegenerative diseases and neurological disorders. Derived from tissue isolation or pluripotent cell differentiation, this protocol outlines methods for culturing and characterizing human or rat NSCs.
