HyClone Dialyzed Fetal Bovine Serum is employed in applications needing serum devoid of low-molecular weight components under Mr 10,000. The serum undergoes dialysis via a diafiltration process, reducing small molecule concentrations like nucleotides and amino acids crucial for alternative biochemical survival paths. Hypoxanthine and thymidine levels are lowered below detection. Unlike traditional dialysis, diafiltration's driving force is hydrostatic pressure, not concentration gradient. Glucose concentration, indicative of dialysis extent, is closely monitored and reported per lot.