Cytiva's HyClone RPMI 1640 medium undergoes rigorous quality control for consistent batches. It's prepared using purified water and filtered through a 0.1 µm filter. Originally designed for long-term culture of peripheral blood lymphocytes, it now serves as a versatile growth medium for various mammalian cell types, including human leukocytes and hybridomas, supporting both suspension and adherent cultures with appropriate supplementation.