Rapid method for quick recombinant virus generation
Powerful polyhedrin promoter for high expression levels
6xHis and V5 tags for easy recombinant protein detection and purification
Versatile Gateway cloning for use with any entry vector
Thymidine kinase gene for nonrecombinant virus elimination
lacZ gene for swift recombinant virus purity check
Established protocol for high-throughput expression
Description
Insect cells facilitate high protein expression with post-translational modifications akin to mammalian cells, easy scalability, and simplified cell growth, adaptable to high-density suspension culture for large-scale production. Many mammalian post-translational modification pathways are present in insect cells, enabling recombinant protein production that's more similar to native mammalian proteins than expression in yeast or other eukaryotes. Baculovirus expression systems are potent and versatile tools for high-level recombinant protein expression in insect cells, with reported expression levels up to 500 mg/L.
