Vary cell concentrations: Aim for 75-150 colonies per dish
Consider multiple cell sources: Progenitors may reside in spleen or bone marrow
Count colonies on the recommended day
Colony morphology may differ based on mouse strain and age
Description
STEMCELL Technologies provides diverse MethoCult methylcellulose-based semi-solid media for mouse cell colony forming unit (CFU) assays. Specific media is crucial for mouse cells to grow distinct colony types, as cytokines in these formulations dictate colony growth. Thus, choosing the right media formulation is vital.
