HotStart Polymerase Action: AptaHotTaq employs DNA-aptamers to block polymerase activity at ambient temperatures, enabling convenient room-temperature PCR reaction assembly
Amplification Capabilities: Up to 5 kb fragments can be amplified using AptaHotTaq. It offers high specificity, producing PCR products free from unspecific products and primer-dimers, suitable for complex genomic and cDNA templates
Buffer Inclusion: The polymerase comes with three different 10x buffers (with and without MgCl2) and additional MgCl2 for universal application
PCR Applications: Standard PCR, Real-Time PCR, Multiplex PCR, suitable for diagnostic applications
IFU Availability: The current IFU is available upon request. Please contact us for details
IFU Download: Click on the pdf icon to download the IFU
Customer Support: If you have any questions about this product or need it customized, please contact us
وصف
AptaHotTaq, a HotStart polymerase, employs DNA-aptamers instead of antibodies to halt polymerase activity at ambient temperatures. These aptamers dissociate reversibly from the enzyme above 50C, enabling convenient room-temperature assembly of PCR reactions that activate during normal cycling. With AptaHotTaq, fragments up to 5 kb can be amplified. The recombinant Taq polymerase offers high specificity, yielding PCR products free of unspecific products and primer-dimers, making it suitable for amplifying complex genomic and cDNA templates. The polymerase comes with three different 10x buffers (with and without MgCl2) and additional MgCl2 for universal application.
