Radial immunodiffusion quantitatively determines the level of an antigen. Antibody is incorporated into liquefied agar and allowed to gel. The antigen is added to small wells and radiates throughout the antibody-containing medium, leaving a precipitate throughout the gel. The amount of diffusion is quantified.
Group Size:
For 10 quantifications 6 reactions each
Time Required:
Incubation overnight
Kit Includes:
Instructions, antigen and antibody, petri plates, pipets, well cutters, agarose, buffer, microtest tubes.
All You Need:
Automatic micropipettes with tips, water bath, microwave or hot plate, incubation oven, laboratory glassware, pipet pumps or bulbs, rulers, paper towels, distilled water.
Storage:
Refrigerator