In this experiment, your students will develop an understanding of bacterial transformation by plasmid DNA by introducing an opportunity to observe an acquired phenotypic trait of the transformed bacterial cells. The presence of blue bacterial colonies visually demonstrates the expression of a specific gene for the Lac+ phenotype.
No IPTG used in this experiment.
Explore genetic engineering, the central dogma, and enzymes in this packed experiment
Transform E.coli with a plasmid DNA containing β-galactosidase and β-lactamase genes
Identify transformed cells based on their blue color and ability to survive on agar with ampicillin
Calculate transformation efficiency and compare results to experimental controls
Group Size:
For 10 lab groups
Time Required:
Complete in 50 minutes and grow overnight
Kit Includes:
Instructions, BactoBeads, plasmid DNA, buffer, media, ampicillin, X-Gal, ReadyPour agar, petri dishes, sterile pipettes, loops and microtubes.
All You Need:
Automatic micropipette (5-50µl) and tips, two water baths (37˚C and 42˚C), incubation oven, thermometer, pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave oven, and hot gloves
Storage:
Some Components Require Refrigerator and Freezer Storage