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Using CRISPR to Treat Cystic Fibrosis for 8 Gels in Saudi Arabia Using CRISPR to Treat Cystic Fibrosis for 8 Gels in Saudi Arabia

Using CRISPR to Treat Cystic Fibrosis for 8 Gels

SAR 742
– 1 +

Special Features

  • Kit Includes: Instructions, Ready-to-Load QuickStrip DNA Samples, UltraSpec-Agarose, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue DNA Stain.
  • Simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis.
  • Develop an understanding of guide RNA (gRNA) design.
  • Use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.
  • Understand the basic biology behind CRISPR technology and explain it's biological basis

Description

In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.
Group Size:
For 8 Gels
Time Required:
Complete in 45 minutes
Kit Includes:
Instructions, Ready-to-Load QuickStrip DNA Samples, UltraSpec-Agarose, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue DNA Stain.
All You Need:
DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.
Storage:
Room Temperature Stable. Storage of Ready-to-Load QuickStrip samples in the refrigerator is recommended

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