Chromaflex chromatography columns have borosilicate glass construction with PTFE end fittings and HDPE bed supports. Each end fitting has a thin PTFE layer that is curled over the O-ring to form a solvent resistant, leak free seal. These columns can be used with all aqueous buffers. To use with organic solvents order optional PTFE bed supports from “Accessories” below. Columns have a temperature range 0 to 50°C; 1/4-28 flangeless fittings. Use ethylene oxide, N NaOH or 100% ethanol for sterilization (autoclaving is not recommended) What's included: each column is supplied with two PTFE end fittings with 20 and micro;m porosity HDPE bed supports, five feet (1.5 m) PTFE tubing, two flangeless tubing nuts and two flangeless ferrules. Jacketed columns PTFE shielded o-rings form a solvent resistant, leak free seal.