The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL LC-NH2-NP HPLC Columns for additional details).. Parameter: =70 °C temp. range. Application(s): HPLC: suitable. 4mm ID and 25cm Length. . . Sigma-Aldrich 58338C40 SUPELCOSIL LC-NH2 HPLC Column, 5 µm Particle Size, 4mm ID, 25cm Length. Country of Origin : United States. Part Number : 1179R69EA. Pack of : 1.