Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using instruments such as the Qubit, SmartDrop XF, and microplate readers. The reagents are highly selective for double-stranded DNA (dsDNA); quantification results will not be skewed by single-stranded DNA (ssDNA), RNA, protein, or nucleotides in a sample. Included with each kit are a concentrated fluorescent assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, sample is added (1 µL to 20 µL), and the concentration determined using an appropriate fluorometer instrument that utilizes blue excitation (460 ± 20 nm) and measures green fluorescence (525-570 nm).

The Accuris High Sensitivity Kit provides an accurate method to quantify low concentration dsDNA samples. The assay kit is designed to be accurate for initial DNA sample concentrations of 0.005–120 ng/µl, providing a detection range of 0.1–120 ng.