Forensic DNA fingerprinting has become a universally accepted crime-fighting tool. Recent advances use the polymerase chain reaction (PCR) to amplify human DNA obtained from crime scenes. This experiment, based on a crime scene scenario, has an inquiry-based component.
Learn about DNA fingerprinting while solving a crime!
Introduce the biotechnologies of restriction enzyme digestion, PCR, and electrophoresis
Perform agarose gel electrophoresis to separate differently-sized DNA molecules
Load, run, and analyze Ready-to-Load PCR reactions obtained from suspects and the crime scene.
Group Size:
For 8 gels
Time Required:
Complete in 45 minutes
Kit Includes:
Instructions, Ready-to-Load QuickStrip DNA Samples, UltraSpec-Agarose, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue DNA Stain.
All You Need:
DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.
Storage:
Room Temperature Stable. Storage of Ready-to-Load QuickStrip samples in the Refrigerator is Recommended.